From amirviz from upo.es Mon Mar 3 06:59:34 2008 From: amirviz from upo.es (Antonio) Date: Mon Mar 3 13:27:10 2008 Subject: [Celegans] Eppendorf Patchman NP2 vs Injectman NI2 equipment for C. elegans injection Message-ID: Dear worm fellows I will appreciate to know whether anyone has compared the efficiency of Eppendorf Patchman NP2 versus the Injectman NI2 equipment for C. elegans injection The price difference is about 3.000 euros and we want the equipment exclusively for C. elegans injection so we initially would go for a Patchman equipment unless someone advices us on the opposite. Thank you very much in advance Looking forward to your reply All the best Antonio /////////////////////////////////////////////////////////////////////// /////////////////////////////// Antonio Miranda Vizuete, PhD. Centro Andaluz de Biolog?a del Desarrollo (CABD-CSIC) Dpto. de Fisiolog?a, Anatom?a y Biolog?a Celular Universidad Pablo de Olavide Carretera de Utrera Km. 1 41013 Sevilla ESPA?A (SPAIN) Tel?fono +34 954 348558 Fax +34 954 349376 email amirviz@upo.es From susana.santos from icfo.es Thu Mar 6 04:56:05 2008 From: susana.santos from icfo.es (Susana Santos) Date: Thu Mar 6 13:12:35 2008 Subject: [Celegans] Lab climate control Message-ID: <20080306105605.rnkv4aszk4wwc4kk@www.icfo.es> Dear colleagues, Our photonics institute (ICFO, Barcelona) is increasing in size and a new biolab is being created. In this lab we will work (within other model organisms and cells) with C. elegans. Currently we are planing the climate control for this lab and I would like to have the best conditions possible for the culture of our soil worms. Can anyone give me specific advise on optimal requirements (in terms of temperature, humidity, air filtration systems, etc.) to work with C. elegans. In case someone has been through the same process, can you give me some advise for effective climate control equipment. Any input (as small as it may seam) will be most welcome. Thank you in advance. Best Susana -- Susana Santos, PhD ICFO-Institut de Ciencies Fotoniques Mediterranean Technology Park 08860 Castelldefels (Barcelona) Spain Tel: +34 93 55 34 050 Fax: +34 93 55 34 000 Email: susana.santos@icfo.es http://www.icfo.es From nash from cshl.edu Mon Mar 24 08:52:31 2008 From: nash from cshl.edu (Nash, Bruce) Date: Mon Mar 24 12:38:28 2008 Subject: [Celegans] Lactose RNAi induction Message-ID: <640CE06B3C0A11429127B2550390A10B2ABB64@mailbox09.cshl.edu> Dear Eric, Have you had continued success using Lactose with NGM-lite instead of IPTG for RNAi induction? I saw a 2003 report that it works well for you and wondered if this has held up since then. Thank you, Bruce Bruce Nash nash@cshl.edu From gshemer from nc.rr.com Fri Mar 28 20:48:05 2008 From: gshemer from nc.rr.com (gidi home) Date: Sat Mar 29 13:07:43 2008 Subject: [Celegans] coating membranes Message-ID: <000a01c8913e$ee79d1e0$0a00a8c0@bio.unc.edu> Hi, I am looking for an efficient way to coat cell surfaces with1-2 micron beads. We have used in the past carboxilated-modified 0.2-1micron beads and they do bind, but I need this binding to be extremely strong and efficient. I was wondering whether anyone is aware of an antibody that recognizes membrane proteins presented on embryonic cells, and if there is any experience with attaching beads to these antibodies. Thanks, Gidi Gidi Shemer, PhD Goldstein lab Dep. of Biology UNC- University of North Carolina at Chapel Hill Phone: (919) 843-8576 Fax: (919) 9621625 Mailing address: CB#3280, Coker Hall, The University of North Carolina at Chapel Hill Chapel Hill, NC 27599-3280, USA