[Celegans] Real Time PCR
g.Joshua from lshtm.ac.uk
(by g.Joshua from lshtm.ac.uk)
Thu Jan 8 07:11:07 EST 2009
Is there a consensus on best/good housekeeping gene in C. elegans to use
to normalise against in Real Time PCR?
Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but
find large differences in RNAs from two worm populations (in all replicates).
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
London WC1E 7HT.
Tel: (+44) (0)20 7927 2028
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E-mail: g.joshua from lshtm.ac.uk
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