Zero-background reporter gene vectors

cam93 cam93 at aber.ac.uk
Wed Dec 1 10:46:07 EST 1993


In article <2de654$15e at hsdndev.harvard.edu>, riyer at rascal.med.harvard.edu says:
>
>
>pow
>Hi netters,  I would like to report the availability of a set of  reporter gene vectors that have some distinct advantages over those  curr
>
> 
>to the fact, that they use a  modified SV40-t-intron as the donor for the poly-adenylation signal.  The wild type SV40-t-intron is the gene
>
>s
> feature was not a serious limitation since scientist  were analysing strong promoters/enhancers that produced high  signal to noise ratios
>
>l
>l as  specificity. The Syncat & pFlash series of vectors provide precisely  these capabilities, since they contain a t-intron polyA signal 
>
>o
>cumented  to produce no spurious interactions with enhancers of interest. By  contrast vectors like pCAT-promoter or pGL-promoter use the S
>
>i
>n our being scooped  by a competing lab.  Other features of these vectors include a very  versatile multiple cloning site upstream and down
>
>r
>egion upstream of the reporter gene ---to---  creation of nested deletion mutant libraries followed by sequence  verification ---to--- ssDN
>
>
Your information might be interesting!, from the tiny bit in the middle
of the screen that can be read!  and where's the rest of it?
do try to think thin and do it again so that we can read it
thanks C.M.M.



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