Golgi-specific antibodies

Martin Snider snider at biochemistry.cwru.edu
Tue Dec 14 11:33:20 EST 1993


In article <2e1to8$n6d at mserv1.dl.ac.uk>, Thorben Lundsgaard
<kvlxthlu at uts.uni-c.dk> wrote:
> 
> 
> Anybody knows a source of antibodies specific for golgi in plant cells
> (monocots)?
> 
> Thorben Lundsgaard
> Dept. of Plant Biology
> Agricultural University
> Copenhagen, Denmark
> kvlxthlu at uts.uni-c.dk 

I don't know of any antibodies specific for monocot Golgi, but I do have an
alternative suggestion--NBD-ceramide.  Richard Pagano at the Carnegie
Institution of Washington developed a staining method using this
fluorescent sphingolipid (Pagano et al., J. Cell Biol. 109, 2067 [1989]). 
NBD-ceramide stains the Golgi in both living and fixed animal cells.  I
don't know if it's been used on plant cells but you might give it a try. 
===================================================================
                           Martin D. Snider
            Department of Biochemistry, School of Medicine
  Case Western Reserve University, Cleveland, OH 44106-4935 USA
              Tel: 216-368-5572    Fax: 216-368-4544
===================================================================



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