apoptosis and cell viability

LOCKSHIN, RICHARD A YPRLBIO at sjumusic.stjohns.edu
Thu Apr 7 09:53:34 EST 1994


mzhou
 asks how to define an apoptotic body
B--
this is tough.  the trypan blue exclusion measures phagocytosed apoptoti
c fragments in vacuoles in macrophages or other cells.  the bodies
themselves are supposed to be impermeable and are identified, during
their short life by highly condensded DNA, in curlure often marginated
and by the condensed nature of the entire structure.  usually there
is no other good marker, though piacentini and fesus have identified
transglutaminase in the bodies.




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