Immunologist seeking job

Andrey Tsouladze tsoul at techunix.technion.ac.il
Sat Apr 23 09:56:42 EST 1994



I am posting this for a friend who does not have an Internet access.  He
had been working for about two years in the lab next to mine. Tigran  is
one of the brightest young scientist I ever  met,  and  a  most  amiable
person. Being in Russia (or Armenia, for that matter) he is wasting  his
talent. This is a real shame.

He is looking for a postdoctoral, or a similar position in cell  biology
or immunology, including clinical immunology. If interested, you can try
and contact him directly. If this proves difficult, reply to my address.

Sincerely,

Andrey

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
*             Dr Andrey Tsouladze               *                   *
*            Department of Biology              * You               *
*  Technion - Israel Institute of Technology    *   have            *
*                Haifa 32000                    *      been         *
*                  Israel                       *         warned... *
*    E-mail: tsoul at aluf.technion.ac.il          *                   *
*    E-mail: tsoul at techunix.technion.ac.il      *                   *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *



                            CURRICULUM VITAE

                            Tigran K. DAVTYAN


Address:                 Laboratory   of   Immunology
                         Institute  of   Epidemiology,   Virology,   and
                         Medical Parasitology
                         Republic of Armenia Ministry of Health
                         21a Gevork Kochar
                         375009 Yerevan, Armenia

Phone:                   +7 8852 56 21 02
Telex:                   243318 ARAKS SU
Fax:                     +7 8852 56 40 30

Born:                    December 1, 1966, Yerevan, Armenia

Family:                  Married, my wife: Asmik Arakelyan, born October
                         7, 1966, Engineer in Physics
                         We have two  daughters:  Araks,  born  May  19,
                         1989, and Arusyak, born September 29, 1992

Citizenship:             Armenian

Highest degree:          Ph.D. (Candidate of Biol. Sci.)
  1993

Research Interests:      Molecular mechanisms of stress  resistance  and
                         immunity, autoimmunity; molecular mechanisms of
                         antigen presentation and  antibody  production,
                         genetic regulation of immune response

Education:
1988                     Graduated from Department of  Pharmacology  and
                         Medical Chemistry, Yerevan Medical Institute

Professional Background:
1988 - present           Research  scientist  in   the   Laboratory   of
                         Molecular and Cellular Immunology, Institute of
                         Epidemiology,     Virology,     and     Medical
                         Parasitology, Armenia Ministry of Health.
                         We work there on the in vitro  immunization  of
                         human peripheral blood lymphocytes for  getting
                         human-mouse  heterohybridomas   which   produce
                         human monoclonal antibodies  against  bacterial
                         toxin and viral proteins. We have obtained some
                         antibody0producing  clones  to  these  antigens
                         which appeared to be rather unstable in respect
                         to their  karyology  and  human  immunoglobulin
                         production.

1988 - 1989              Senior  technician   in   the   Laboratory   of
                         Molecular and Cellular Immunology, Institute of
                         Molecular Biology, Acad.Sci.Armenia.
                         During this  period,  I  had  been  working  on
                         establishing mouse hybridomas.

1989 - 1992              Research  scientist,  Laboratory   of   Genetic
                         Mechanisms   of    Cell    Malignization    and
                         Differentiation  headed  by  Dr   T.N.Ignatova,
                         Institute of Cytology, St.-Petersburg, Russia.
                         During  my  stay  in  this  institute,  I   was
                         involved  in  the  project  of  multiple   drug
                         resistance  of  hybridoma  and  myeloma  cells.
                         Cells selected for ethidium bromide  resistance
                         were shown to produce more immunoglobulins  and
                         have their MDR1 gene amplified.  Both  selected
                         and unselected  cells  synthesize  and  secrete
                         much more  immunoglobulins  when  treated  with
                         EtBr or adriamycin. Adriamycin  stimulates  the
                         synthesis of immunoglobulins via  free  radical
                         production of hybridoma cells.
                         Also, we  have  shown  that  these  drugs  bind
                         immunoglobulins inside and  outside  the  cell.
                         Analogous data were obtained using normal human
                         lymphocytes. These  data  made  me  state  that
                         there exist an additional mechanism  (different
                         from MDR-mediated) of drug efflux from the cell
                         via immunoglobulin secretion.

1993                     I have got my Ph.D. in Genetics and  Immunology
                         at the Dept of Genetics and Cytology of Yerevan
                         University. It is entitled "Induction of Immune
                         Response of Human lymphocytes  in  vitro".  The
                         supervisors of my Ph.D. work are: Corresponding
                         Member of the  Natl.Acad.Sci.Armenia,  Director
                         of the Institute of Epidemiology, virology, and
                         Medical parasitology (Yerevan),  Prof  Yuri  T.
                         Alexanyan,  and  Head  of  the  Laboratory   of
                         Genetic Mechanisms of  Cell  Malignization  and
                         Differentiation of the Institute  of  Cytology,
                         Acad.Sci.Russia (St.-Petersburg), Prof  Tatyana
                         N. Ignatova.

Methods:                 Various   variants   of   immunoenzyme   assay,
                         immunoblotting, gel electrophoresis,  isolation
                         and purification of DNA, RNA, and proteins from
                         cells,  human  cells  separation,  cultivation,
                         hybridoma   generation,   and   production   of
                         antibodies.


                              PUBLICATIONS

1. T.K.Davtyan, B.Ch.Nisman, Yu.T.Alexanyan,  and  T.N.Ignatova  (1991).
   The  possibility  of  producing  the  human  monoclonal  antibody  by
   immunization of human peripheral blood lymphocytes in vitro. Biol. J.
   Armenia 44, No.2, p.115.

2. M.B.Melixetyan, T.K.Davtyan, E.V.Beriozkina, T.N.Ignatova, and  Yu.T.
   Alexanyan (1991). The effect of adriamycin and  ethidium  bromide  on
   the antibody production by mouse B-cell hybridomas. Biol. J.  Armenia
   44, No.2, p. 120.

3. T.K.Davtyan, G.I.Blinova, Yu.T.Alexanyan,  and  T.N.Ignatova  (1991).
   Induction of long-term proliferation of normal human  lymphocytes  by
   co-cultivation with ethidium bromide resistant L929  lines  of  mouse
   transformed fibroblasts. Biol. J. Armenia 44, No.2, p. 155.

4. T.K.Davtyan, B.Ch.Nisman, T.N.Ignatova,  and  Yu.T.Alexanyan  (1991).
   The effect of L-leucin methyl ester on  the  functional  activity  of
   human peripheral blood lymphocytes in vitro.  Biol.  J.  Armenia  44,
   No.3, p. 235.

5. Yu.T.Alexanyan, and T.K.Davtyan  (1991).  The  problem  of  producing
   human monoclonal antibodies. Immunologia, No.3, p. 10.

6. T.K.Davtyan, M.B.Melixetyan, T.N.Ignatova, and Yu.T.Alexanyan (1993).
   Adaptive response of  B-cells  to  cytotoxic  drugs  in  culture.  1.
   Adriamycin and ethidium bromide stimulate the synthesis and secretion
   of immunoglobulins by hybridomas and lymphocytes. Tsitologia 35,  No.
   4, p. 54.

7. T.K.Davtyan, M.B.Melixetyan, Yu.T.Alexanyan, and T.N.Ignatova (1993).
   Adaptive response of B-cells to cytotoxic drugs in  culture.  2.  The
   interaction of adriamycin and ethidium  bromide  with  immunoglobulin
   molecules. Tsitologia 35, No.6/7, p. 91.

8. M.B.Melixetyan,   T.K.Davtyan,   I.V.Ivanova,   Yu.T.Alexanyan,   and
   T.N.Ignatova (1993). Adaptive response of B-cells to cytotoxic  drugs
   in  culture.  3.  A  possible  mechanism  of  adriamycin  action   on
   immunoglobulin production by mouse hybridoma  cells.  Tsitologia  35,
   No.6/7, p.98.

9. T.K.Davtyan, T.B.Smirnova,  Yu.T.Alexanyan,  T.N.Ignatova,  and  M.B.
   Melixetyan (1993). The interaction  of  cultivated  human  peripheral
   blood lymphocytes with mouse  transformed  fibroblasts.  Immunologia,
   No.3, p.17.

10.T.K.Davtyan (1993). Induction of Immune Response of Human lymphocytes
   in vitro. Ph.D.  thesis.  Dept  of  Genetics  and  Cytology.  Yerevan
   University.



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