Tyrosine Hydroxylase antibodies

Richard Burge R.Burge at bay.cc.kcl.ac.uk
Fri Apr 29 16:38:45 EST 1994

I'm having no end of trouble trying to stain up some cultures of ventral 
mesencephalon/substantia nigra with a rabbit anti-tyrosine hydroxylase 
antibody from Chemicon. I was wondering if anyone could recommend 
any good antibodies to TH for immunocytochemistry. I have seen papers staining 
TH positive cells after fixation in paraformaldehyde fixative with no 
glutaraldehyde, and was wondering if perhaps TH is sensitive to glutaraldehyde.

Any advice would be much appreciated.

Richard Burge         | e-mail:
King's College London |  R.Burge at bay.cc.kcl.ac.uk

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