Lipid Label Fixation Advice

lowy at vax.afrri.usuhs.mil lowy at vax.afrri.usuhs.mil
Mon Aug 8 06:41:42 EST 1994



Does anyone have a favorite fixation method for light level
microscopy to preserve the cellular distribution of  fluorescently
labeled lipid analogues?  I've tried some (but not all) of the
standard immunocytochemical fixes and they result in either 
extraction or redistribution of my labels. The labels are generally
in the plasma membrane. Post or email ok.

Thanks in advance

Joel Lowy

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Joel Lowy				The usual disclaimers.
Physiology Dept.
AFRRI, 8901 Wisconsin Ave., Bethesda, MD 20889-5603, USA 				 	
lowy at vax.afrri.usuhs.mil
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