Isolated cell disruption methods?

Fergus Doherty mbxfd at unicorn.nott.ac.uk
Wed Aug 10 09:10:17 EST 1994


Hello,
Can anyone give advice on the relative merits of:

* the nitrogen cavitation bomb
* the 'cell disrupter' from the EMBL workshop (a steel block wih a narrow 
channel with a ball bearing in it around which a cell suspensio is forced
with a syringe)
* the 'mini bead-beater' (a device for violently agitating a cell
suspension with glass beads)
*  any other method

for disrupting isolated cells (ie culture cells) to obtain homogenates
suitable for isolation of intact organelles (ie lysosomes)?

If you know of the EMBL cell disrupter can you tell me who to contact there
(e-mail if possible) or if there is another source for this device.  Any
other recommendations appreciated, bearing in mind the cells in questions
are small (monocytes, RBL cells and reticulocytes) and have proven
difficult/impossible to homogenize by other methods (eg Dounce).

Fergus Doherty,
dept. Biochemistry,
University Medical School,
Queen's Medical Centre,
Nottingham NG7 2UH
Tel: 602 709366  FAX 602 422225 Internet: mbxfd at unicorn.nott.ac.uk



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