Antibodies to phsophoserine and phosphothreonine

[anthonyp at scripps.edu]

In article <32e5h5$qns at news.duke.edu>, <Gliksman at uncvx1.oit.unc.edu> writes:

> Has anybody had good experience with antibodies toward phosphoserine or
> phosphothreonine?  I believe Sigma is selling some but they are not
> cheap and I am wondering if people have had good experinces using them
> on a variety of proteins.
> 
> -Neal Gliksman


I'd have to say I have not had good experience with them.  I've done more 
western blots than I care to count over the last 5 years, most of the 
phosphotyrosine.  I have used several mAbs to that.  
I have not given up on the antiphosphoser and antiphosphothre, but they are a 
bit finicky.  Lots of background problems.

-tony