WEHI (b cell) tranfections - lack of expression
steven joseph chmura
sjchmura at kimbark.uchicago.edu
Thu Aug 18 10:50:05 EST 1994
This is really quite odd, and I am at a loss for an explanation.
We have been transfecting WEHI231 cells which are an imature murine
b cell lineage with various constructs. We have used CMV-neo-X,
We select for the neo resistant strains - works great.
But then we look for expression of our protein - nothing.
Look at northern's - nothing.
We run PCR - we have the gene there.
What is going on? Is the prmoter being turned off? Am I
just incapable of running a western?
Has anyone else had similiar difficulties with b cell lineages?
________________________________________________________________________________Steven Chmura University of Chicago Medical School(MSIII,PhD1)
"If medicine was easy, any idiot would do it"
"Morality is simply the attitude we adopt toward people whom we dislike"
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