Freezing cells??

Colin Rasmussen ras-1 at bones.biochem.ualberta.ca
Fri Dec 9 23:04:57 EST 1994



On 9 Dec 1994, Marc Stein wrote:

> I don't know about planaria, but when we freeze mammalian cells,
> we include 10-20% DMSO in the medium, to prevent ice-crystals.
> We also do as Robin suggests, insulating the sample with styrofoam
> or tissue paper for a slow freeze in a -70 freezer.
>  
We freeze in 90% fetal calf serum, 10% DMSO, we put them in the fridge 
for 1 hour, at -70C for 1 hr, then into liquid nitrogen.

Colin



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