Cell proliferation techniques

ksasaki at gan.ncc.go.jp ksasaki at gan.ncc.go.jp
Wed Dec 14 07:49:08 EST 1994


Hello Mr.GUIVARC_H Dominic,

>Help! I need some information about techniques who could measure the
>proliferation of cells where in suspension. Thanks for help.


Try AlamrBlue; you just apply the dye to a culture well of your
interest (final 10%) and incubate it for a certain period, then 
spin down the cells and measure the absorbance of supernatants.  
It works fine. No need for extraction steps, as with MTT dye assay.

Hope this helps.

Sorry for my poor English.

K.SASAKI, M.D.
National Cancer Center, Tokyo, Japan




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