Synthetic Peptide Antibody Questions

John D. Valentich shark at sam.neosoft.com
Tue Dec 20 13:00:11 EST 1994


We have recently started preparing polyclonal antibodies in rabbits against synthetic peptides 
from annexins and phospholipase A2 activating protein (PLAP).   Peptides were 
conjugated to the carrier BSA.  We are wondering about the problem of 
antibodies generated against BSA reacting with serum albumin or albumin-like 
epitopes in other cellular proteins.  Questions:

1.  Is it likely (or inevitable) that our antisera will contain high titer antibodies against BSA? 
Could they be expected to cross react with other non-albumin cellular proteins?

2.  If so, is the best (or only?) solution to this problem affinity 
purification of annexin or PLAP antibodies from the antisera?

3.  What are other possible solutions?



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