Andrew K. Groves
grovesa at starbase1.caltech.edu
Wed Dec 28 13:17:40 EST 1994
In article <1994Dec28.121316 at earth.medcolpa.edu>,
osborneaj at earth.medcolpa.edu wrote:
> Need helful hints on how to grow microvascular endothelial cells. Any
> do having hard time getting cells to become confluent. Is there some kind of
> magic dust that I could sprinkle to get them to grow, and keep down
> contamination? Please, once again any help will do.
An 'overkill' cocktail which should keep them happy is 15-20% serum,
10ng/ml basic FGF, plus heparin. The serum will also help keep
contamination down. Grow the cellks on either home-made collagen or the
fancy commercially available stuff.
Division of Biology, 216-76
California Institute of Technology
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