recovery fn after electroelution

wiens at cobra.uni.edu wiens at cobra.uni.edu
Thu Jan 6 12:20:10 EST 1994


I wonder what is the best method to retrieve native function of a protein
electro-eluted from a gel.  Can one do better than dialysis followed by
lyophilization, solubilization in water, and then precipitation in
methanol-acetone (as described in Current Protocols)?  I would like to
reconstitute electroeluted membrane channel proteins for insertion into
artificial phospholipid bilayers, but I worry about the SDS. 
                                 



More information about the Cellbiol mailing list