recovery fn after electroelution
wiens at cobra.uni.edu
wiens at cobra.uni.edu
Thu Jan 6 12:20:10 EST 1994
I wonder what is the best method to retrieve native function of a protein
electro-eluted from a gel. Can one do better than dialysis followed by
lyophilization, solubilization in water, and then precipitation in
methanol-acetone (as described in Current Protocols)? I would like to
reconstitute electroeluted membrane channel proteins for insertion into
artificial phospholipid bilayers, but I worry about the SDS.
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