recovery fn after electroelution

wiens at wiens at
Thu Jan 6 12:20:10 EST 1994

I wonder what is the best method to retrieve native function of a protein
electro-eluted from a gel.  Can one do better than dialysis followed by
lyophilization, solubilization in water, and then precipitation in
methanol-acetone (as described in Current Protocols)?  I would like to
reconstitute electroeluted membrane channel proteins for insertion into
artificial phospholipid bilayers, but I worry about the SDS. 

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