hollow fibre bioreactors

Robert Burns burns at sasa.gov.uk
Thu Jul 7 06:24:20 EST 1994


Does anybody out there have experience in maintaining hybridomas
in hollow fibre bioreactor systems. We have a mini-mouse system
and have had a lot of bother getting the cells to colonise the
fibres properly.

We have one cell line growing at present which has been in the
cartridge for about six weeks and was giving about one mg of ab
every two days. This has now decreased to about half a mg every
two days. Is this to be expected? 




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