transcription of retrotransposon ?

Bernhard Seehaus dh30 at pop.th-darmstadt.de
Thu Jul 7 02:12:44 EST 1994


Dear collegues,

Im working towards my PhD and doing research on the differentiation of
certain eucaryotic cells -:). After a subtractive cloning step and 
following differentiel hybridzation I isolated about ten more or less cell
type-specific cDNA clones.
One clone examined by northern blot (mRNA) and pcr showed strikingly high
specifity.
Comparison with databases (genbank and swissprot) (for me) surprisingly
shows very high homology to orf1 of the human LINE-1 sequence, and the
determined length of the transcript also fits.

Is anybody out there who has any idea about that? Especially if there are
any examples of cell type-specific transcription of retrotransposons? Do
you think it is probably a pseudogene without a gene product?

Any help is appreciated, thanx in advance


Bernhard Seehaus
Institut of biochemistry
University of Darmstadt
Germany
e-mail dh30 at pop.th-darmstadt.de



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