source of collagen-coated dishes
olam at radium.uio.no
Tue Jul 19 05:06:19 EST 1994
In article <gy13-180794132039 at 18.104.22.168>, gy13 at cornell.edu (Ge Yang) wrote:
> Does anyone out there know about any good source of collagen-coated dishes
> which are used for cell culture?
You may make these yourself:
Dissolve collagen to 5 microgram in 0.2 % acetic acid.
Add 2 ml per 6 cm dish, let stand 1 min, pour off, and let dry.
The dish has been coated.
The details are in Gjessing and Seglen Exp Cell Res 129:239-49, 1980.
Your have to select the right type of collagen for your cell type.
Ola Myklebost Email ola.myklebost at labmed.uio.no
Dept of Tumor Biology
Inst for Cancer Research Tel +47-2293-4299
The Norwegian Radium Hospital Fax +47-2252-2421
N-0310 OSLO, Norway
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