source of collagen-coated dishes

Ola Myklebost olam at radium.uio.no
Tue Jul 19 05:06:19 EST 1994


In article <gy13-180794132039 at 132.236.30.70>, gy13 at cornell.edu (Ge Yang) wrote:
> Does anyone out there know about any good source of collagen-coated dishes
> which are used for cell culture?

You may make these yourself:

Dissolve collagen to 5 microgram in 0.2 % acetic acid.

Add 2 ml per 6 cm dish, let stand 1 min, pour off, and let dry.

The dish has been coated. 

The details are in Gjessing and Seglen Exp Cell Res 129:239-49, 1980.

Your have to select the right type of collagen for your cell type.

-- 
Ola Myklebost                   Email  ola.myklebost at labmed.uio.no
Dept of Tumor Biology
Inst for Cancer Research        Tel +47-2293-4299
The Norwegian Radium Hospital   Fax +47-2252-2421
N-0310 OSLO, Norway



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