ES selectable markers
mkennedy at chmeds.ac.nz
Tue Mar 1 16:46:28 EST 1994
In article <fodde.2.000E9C75 at ruly46>, fodde at ruly46 (Riccardo Fodde) writes:
> I'm looking for a dominant selectable marker to transfect an ES cell line
> which is already G418 and Hygromicin resistant. Can anybody help me?
> Any suggestion will be very much appreciated.
> Riccardo Fodde
There are others you could use, though I haven't tried anything other than
hygro and G418 in ES cells. HPRT minigenes have been used successfully in ES
Reid et al., 1990. PNAS 87, 4299-4303
Magin et al., 1992. Gene 122, 289-296
van der Lugt et al., 1991. Gene 105, 263-267
Other markers that may be useful (though I'm not aware whether they've been
used in ES cells) are:
MDR1 Kane et al., 1989. Gene 84, 439-446.
pSV2his, pSV2trp Hartman & Mulligan, 1988. PNAS 85, 8047-8051.
There are others around I'm sure, but obviously you want to be careful what
sort of selection you apply to your ES cells, or you may cause them to
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