expression in E.coli

[Mark Bond]

Does anyone have any suggestions as how to maximize eucayotic gene expression 
in E.coli.  I have been trying to induce expression of a GST/talin fusion 
protein and have only been able to produced small amounts of protein.  I know 
that at 37oC many foreign protein in E.coli aggregate and this can be 
partially overcome by incubating the cells at a lower temperature.  Is this 
the only method that I can use to incease the protein yield.