expression in E.coli

Mark Bond plyhmb at pln1.nott.ac.uk
Wed Mar 16 13:12:27 EST 1994


Does anyone have any suggestions as how to maximize eucayotic gene expression 
in E.coli.  I have been trying to induce expression of a GST/talin fusion 
protein and have only been able to produced small amounts of protein.  I know 
that at 37oC many foreign protein in E.coli aggregate and this can be 
partially overcome by incubating the cells at a lower temperature.  Is this 
the only method that I can use to incease the protein yield.



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