expression in E.coli

Anne Savitt asavitt at
Wed Mar 16 13:08:40 EST 1994

My suggestion is that you Gopher over to the IUBio Biology Archive at
Indiana University and search the Bionet archive for GST and protein
expression - these questions have been thrashed out many times.  There
are many sources for the problems you are experiencing - including
insoluble proteins, protease problems, codon usage problems, internal
ribosome binding sites, etc.  There!s a wealth of information in the
archive which will get you started sorting it out.

Good luck.

Anne Savitt
Department of Microbiology
SUNY at Stony Brook
Stony Brook, NY   11794-5222
email:  asavitt at

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