glutaraldehyde fixation and biotin

Anne Spang spang at vms.biochem.mpg.de
Sun Nov 6 13:21:29 EST 1994


Hi, 
You could use LR-White as embedding resin. This resin allows you to do
immunostaining, while according to my experience, Epon is not working. I
can only give you a reference for yeast cells, but perhaps you can use a
similar protocol for your cells. 
Preuss, D., J. Mulholland, A. Franzusoff, N. Segev, and D. Botstein
Molecular Biology of the Cell Vol 3. 789-803, July 1992 

Hope this helps!
Anne



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