Q RE: Equine serum

[Jason at UofSask]

Hi there,
	Does anyone have any experience using equine serum for 
endothelial cell (EC) cultures?  I read that it apparently has 
good as well as repeated success with stimulating EC growth while 
inhibiting the proliferation of other cell types (non-factor VIII related 
cells) in culture.
	I am currently using 15-20% calf serum for my ECs (from bovine 
brain capillaries) with limited success (contaminating cell types other 
than ECs are a problem).  Any suggestions for a good way of eliminating 
or limiting the growth of non-endothelial cell types in culture are also 
welcome. 
	Thanks in advance for your help.     

Jason Ram
Vet. Physiol. Sci.
U of Saskatchewan
Canada