Luciferase detection

Martin Leach leach at mbcrr.harvard.edu
Wed Nov 23 11:37:12 EST 1994


In article <1994Nov23.141230.3409 at genes.icgeb.trieste.it>,
enrico at genes.icgeb.trieste.it (Enrico Tongiorgi) wrote:

> Hallo,
> 
> I have transfected HeLa cells with a vector having as a reporter gene
> the Luciferase gene. Is it possible to add substrates (D-luciferine etc.)
> to the medium in oder to detect the cells which express the reporter gene ?
> Does cells survive this procedure ? Has anyone a protocol for this ?
> I thank very much those who wants to help me by sending a mail to:
> TONGI at SISSA.IT
> 
> Thanks from
> Enrico (Trieste, Italy)

use potassium salt of beetle luciferin

i have details somewhere that i tried on neuronal cells...give me time to
dig it up/harass me for it

martin


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.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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