Luciferase detection

Martin Leach leach at
Wed Nov 23 11:37:12 EST 1994

In article <1994Nov23.141230.3409 at>,
enrico at (Enrico Tongiorgi) wrote:

> Hallo,
> I have transfected HeLa cells with a vector having as a reporter gene
> the Luciferase gene. Is it possible to add substrates (D-luciferine etc.)
> to the medium in oder to detect the cells which express the reporter gene ?
> Does cells survive this procedure ? Has anyone a protocol for this ?
> I thank very much those who wants to help me by sending a mail to:
> Thanks from
> Enrico (Trieste, Italy)

use potassium salt of beetle luciferin

i have details somewhere that i tried on neuronal cells...give me time to
dig it up/harass me for it



.....          Martin Leach                Email:leach at 
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