Glutaraldehyde autofluorescence
William Meikrantz
meikrant at fas.harvard.edu
Mon Nov 28 10:29:49 EST 1994
Is there any means of eliminating (red) autofluorescence following
fixation of HeLa cells? I fix for 5 min with 1% EM grade glutaraldehyde
in PBS. I need to preserve beta galactosidase activity which is being
transiently expressed in these cells. If anyone has any experience in
this area I'd love to hear from them.
Thanks,
Bill Meikrantz
meikrant at fas.harvard.edu
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