Glutaraldehyde autofluorescence

William Meikrantz meikrant at
Mon Nov 28 10:29:49 EST 1994

Is there any means of eliminating (red) autofluorescence following 
fixation of HeLa cells?  I fix for 5 min with 1% EM grade glutaraldehyde 
in PBS.  I need to preserve beta galactosidase activity which is being 
transiently expressed in these cells.  If anyone has any experience in 
this area I'd love to hear from them.
Bill Meikrantz
meikrant at

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