Glutaraldehyde autofluorescence

Julie Theriot theriot at wi.mit.edu
Mon Nov 28 15:27:09 EST 1994


In article <3bct1d$9sk at decaxp.harvard.edu>
meikrant at fas.harvard.edu (William Meikrantz) writes:

> Is there any means of eliminating (red) autofluorescence following 
> fixation of HeLa cells?  I fix for 5 min with 1% EM grade glutaraldehyde 
> in PBS.  

Yes, quench with a strong reducing agent such as sodium borohydride. 
E-mail me if you want the detailed protocol.
Julie Theriot
theriot at wi.mit.edu



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