Actin purification

William H. Guilford guilford at salus.med.uvm.edu
Wed Oct 5 07:08:23 EST 1994


In article <Pine.3.89.9409271327.A13042-0100000 at druid>, carter at DRUID.HSC.COLORADO.EDU ("Royston E. Carter PhD") says:


>I need help on methiods for the purification of actin.  
>There are a few points about which I am not clear:
>1) How fresh does the tissue have to be?

  You need to _obtain_ the tissue fresh (like at slaughter), but if you freeze
  it immediately you can still use it later.

>2) Can I make acetone powder from frozen tissue?

  Yes.

>3) Can I store purified actin as a lyophillized powder, or frozen at -70 C?

  Don't know.  It would be better to stick with a storage buffer with the
  actin at relatively high (say around 50mg/ml) concentration.  We've kept
  actin in this form for many months without noticable degradation.


Hope this helps!  

- Bill


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