William H. Guilford
guilford at salus.med.uvm.edu
Wed Oct 5 07:08:23 EST 1994
In article <Pine.3.89.9409271327.A13042-0100000 at druid>, carter at DRUID.HSC.COLORADO.EDU ("Royston E. Carter PhD") says:
>I need help on methiods for the purification of actin.
>There are a few points about which I am not clear:
>1) How fresh does the tissue have to be?
You need to _obtain_ the tissue fresh (like at slaughter), but if you freeze
it immediately you can still use it later.
>2) Can I make acetone powder from frozen tissue?
>3) Can I store purified actin as a lyophillized powder, or frozen at -70 C?
Don't know. It would be better to stick with a storage buffer with the
actin at relatively high (say around 50mg/ml) concentration. We've kept
actin in this form for many months without noticable degradation.
Hope this helps!
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