Protoplasts from immature embryos?

STIFF at wsuvm1.csc.wsu.edu STIFF at wsuvm1.csc.wsu.edu
Thu Oct 13 00:12:44 EST 1994


I'm looking for a protocol to isolate protoplasts from immature embryos
of barley (not from the IE derived callus).  Anybody got any suggestions?
Since these are a real pain to isolate, I was hoping to find an existing
protocol rather than develop one by trial and error.  Thanks for your
help.



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