Animal cell culture assistance
KB at strolchi.swb.de
Thu Oct 13 16:22:00 EST 1994
Bill Scholz wrote:
> Can anyone suggest a good animal (non-human) cell line that grows as a
> suspension without the need for a CO2 incubator? I am looking for
> something that undergraduates with good sterile technique could work with.
how about any transformed lymphocyte T-cell line? As I remember
lymphocytes grow very well in L15 media (supplemented with HEPES,
glutamine, penicilline/streptomycine, and fetal calf serum) without any
CO2-incubation. Concerning to the cell line lymphocytes grow rather rapid
(the cell lines I have once worked with - long ago- doubled cell count
within 1 to 2 days).
Hope I could help,
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