immunofluorescence background

Danny Altshuler altschul at PROBLEM_WITH_INEWS_GATEWAY_FILE
Sun Apr 2 15:36:39 EST 1995

Hi, everybody
I'm doing some fluorescence using the biotynilated 
antibodies/streptavidin Texas Red. I'm using 4% parafolmadehyde and 0,5% 
Triton for the fixation /permeabilization steps.
My problem is in the background. I started using BSA 3% whitout luck and 
then switch to 5% calf serum. The background was reduced but is still there.
Please any suggestion or good protocols are wellcome
BTW these are NIH 3T3 cell lines

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