PI-PLC

Dr. Sharon Powell POWELL at ZOOL.UMD.EDU
Wed Apr 5 13:35:49 EST 1995


    Has anyone out there ever used PI-specific phospholipase C to 
digest GPI-anchored proteins off the surface of living cells?  I 
vaguely remember having tried it in regular culture medium, but 
several references suggest that the enzyme is inhibited by calcium 
and magnesium.  My cells don't like calcium, magnesium free PBS very 
much-any suggestions for conditions?
-Sharon K. Powell
Sharon K. Powell
Assistant Research Scientist
Department of Zoology
University of Maryland, College Park

Voice: 301-505-0266
E-mail: powell at zool.umd.edu












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