Swiss 3T3 after serum starvation
Sin Wun Chey
mcbsinwc at LEONIS.NUS.SG
Thu Apr 20 20:05:32 EST 1995
Hello everyone,
I hope someone can tell me what I saw.
When Swiss 3T3 cell was serum starved from 2 days, there was a ring of
non-transparent material(under ordinary light microscope) around the
nucleus. It had multiple projections and this star-like structure can
extend throughout the cell as though forming a network, stopping short of
the plasma membrane. It is definitely not actin or tubulin (from
immunostaining).
In contrast, a cell happily in serum containing medium does not have this
aggregation and the cytoplasm appears homogenous under low power light
microscope.
I am not a cell biologist and would be grateful for any answers/suggestions.
Thanks.
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