help: cytospins from mouse hematopoietic cells
you at somehost.HSC.Colorado.edu
Mon Aug 14 18:12:56 EST 1995
In article <h-petrie-0208950847590001 at mac-93.k-g2.ski.mskcc.org>, h-petrie at ski.mskcc.org (Howard T. Petrie) says:
>We would like to make good cytospins from mouse hematopoietic cells
>(mainly lymph node, bone marrow, and blood) for morphology and
>immunohistochemistry. Some years back, I used to do this with human cells
>with good results, but now our slides appear uneven in distribution, and
>the cells themselves are distorted. Does anyone have any insights (or a
>methodological reference) as to which parameters are critical, i.e.,
>speed, time, sample volume, sample concentration, serum concentration,
>etc.? Responses can be posted here or sent by e-mail to
>h-petrie at ski.mskcc.org. Thanks.
All those factors are important: but I would first try limiting the rotor
speed to generate 500 x g or less and spin for no more than 3-4 min.
Also, try to limit the volume to about 100 microliters.
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