Immunofluorescence advice needed!

David Jackson djack at bu.edu
Wed Aug 23 10:16:24 EST 1995


Your negative controls will tell the story many times.
Especially the pre-immune rabbit serum.  Try that alone
and see what the background is.  If it's high, then use
a higher dilution of your antiserum and extend your
washes both in # and in time until you optimize.  The
key is minimize non-specificity w/o sacrificing too
much of your specific binding which can only be set by
doing the empirical studies.

David Jackson
djack at bu.edu				E= mc2 
Vit D Lab		It's not just a good idea, it's the law!
BUMC




More information about the Cellbiol mailing list