Immunofluorescence advice needed!
djack at bu.edu
Wed Aug 23 10:16:24 EST 1995
Your negative controls will tell the story many times.
Especially the pre-immune rabbit serum. Try that alone
and see what the background is. If it's high, then use
a higher dilution of your antiserum and extend your
washes both in # and in time until you optimize. The
key is minimize non-specificity w/o sacrificing too
much of your specific binding which can only be set by
doing the empirical studies.
djack at bu.edu E= mc2
Vit D Lab It's not just a good idea, it's the law!
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