Electroporation - help!!

me116 at sdcc1.ucsd.edu me116 at sdcc1.ucsd.edu
Fri Aug 25 00:00:22 EST 1995


In article <01HUEVADG9869VW2OO at vax1.dcu.ie>, 92700080 at VAX1.DCU.IE writes:
>Can anyone help me - I need electroporation conditions for the following 
>cell lines
>K652
>Hut-78
>Hep2
>SW480
>Thanks - Carol.

We electroporated Hut-78 in 300 ul serum-free medium at 250 Volt and
960 uF with the Bio-Rad electroporator. Everything at room temperature
and straight forward without incubating the electroporation mix before
or after the shot.
Optimal conditions have to be determined seperately for every cell line.

Marianne Ostermayer





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