John A. Newitt
newitt at nih.gov
Wed Aug 30 15:01:59 EST 1995
In article <421s3v$eu4 at oban.cc.ic.ac.uk>, "M.Patel" <M.Patel at ic.ac.uk> wrote:
> I am looking for a simple histological stain to show nuclei of
> fixed cultured cells. I can think of a fluorescent stain, Hoechst 33258,
> but would prefer to use an ordinary light microscope.
Why not use the classic hematoxylin and eosin stain? The hematoxylin
stains highly-negatively charged molecules, so you'd get prominent nuclear
staining from hematoxylin binding to the DNA and RNA in the nucleus.
John A. Newitt, Ph.D. | <newitt at nih.gov>
National Institutes of Health | FAX: 301-402-0387
Bethesda, Maryland USA | <this space for rent>
More information about the Cellbiol