labelling oxidised-LDL

john white j.white at lhmc.ac.uk
Mon Dec 11 11:42:12 EST 1995


I'm a chemist, but need to do some cell culture experiments - measuring 
uptake of oxidised-LDL by human macrophage cultures.

Most people seem to label LDL with 125I, then oxidise, then feed. I would 
rather do it using a fluorescence method: e.g. prepare DiI-LDL, then 
oxidise; or ox-LDL then label. BUT I can't find any references to people 
preparing fluorescently-labelled oxidised LDL. Has it been done before, & 
if not why not? 

John




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