(no subject)

Mike Holbrook holbrook at mcb.umass.edu
Tue Dec 12 14:45:51 EST 1995


I am curious about whether or not detergents go bad after extended 
periods of time in buffers at a low concentration.  Specifically, we 
have a 1.0% triton-100 solution (Hepes buffer) which does not seem to be 
solubilizing cell membranes as well as it once did.  The solution is 
about 6 months old and I did not immediately suspect a detergent 
problem.  Any comments would be appreciated.

Thanks,  Mike





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