chromomycin

[Steve Rogers]

In article <4ascum$fed at mail.llu.edu>, bLangridge at ccmail.llu.edu (Bill
Langridge) wrote:

*** We are staining chromosomes for cell sorting. Can anyone tell me how 
*** long one can keep a chromomycin A3 stock solution? It goes into 
*** solution slowly and therefore makes it difficult to plan experiments on 
*** short notice if one must make the stock soln fresh each time.
*** 
*** Thanks for your help!
*** 
*** Bill Langridge

Hi Bill,
I dissolve chromomycin (from Sigma) in PBS + 5 mM Mg to 100 mcg/mL. 
Aliquots are then stored at -20 for up to a year+  with no loss of
staining.  Hope this helps.
Steve

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Steve Rogers
Dept. of Cell & Structural Biology and the
Beckman Institute - Optical Visualization Facility
Univ. of Illinois @ C-U
srogers at delphi.beckman.uiuc.edu
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