Determination of on-rate and off-rate

[George A. Heavner]

I am working with a small peptide (MW < 500) that binds to a specific 
receptor. I would like to determine both the on-rate and off-rate of the 
peptide with the receptor.  Both the purified receptor as well as cell lines 
that express the receptor are available.  I would appreciate suggestions or 
references on procedures that can be used to determine on- and off-rates.

Thanks in advance.

George A. Heavner
Senior Director, Peptide R&D
Centocor, Inc.