niman at vms.cis.pitt.edu
niman at vms.cis.pitt.edu
Fri Feb 10 19:27:32 EST 1995
In article <3hb70h$djn at charm.magnus.acs.ohio-state.edu>, tchang at magnus.acs.ohio-state.edu (Tien-Hsien Chang) writes:
> We have been waiting for Berkeley Antibody Co. to deliver rabbit anti-HA
> antibodies for the past 2 months. The most recent information we got is that
> we still have to wait for them to complete their assessment of the new batch.
> We are aware of BMB is selling mouse anti-HA monoclonal. However the price is
> exceedingly expensive. One gets 200 micrograms for more than $200. And the
> recommended conc. for Western is 10 micrograms per ml.
> Is there a noncommercial source (in academics) where we (and most likely
> many others) can obtain anti-HA monoclonal antibodies (mouse or rabbit)? We
> are interested in using these for immunolocalization studies for HA-tagged
> proteins in yeast Saccharomyces cerevisiae.
> We would appreciate that someone can provide useful suggestions an/or
> protocols regarding using anti-HA antibodies for this purpose in yeast. I
> heard that anti-HA recognizes some related proteins in yeast cells. Is there a
> way to get rid of these signals (we know of preadsorption by yeast powder may
> help)? Pointers to references of using this approach in yeast are greatly
> Tien-Hsien Chang
> Assistant Professor
> Department of Molecular Genetics
> The Ohio State University
> 484 West 12th Ave.
> Columbus, OH 43210
> (614)-292-0631 (phone)
> (614)-292-4466 (fax)
> chang.108 at osu.edu
Tien, I,m not sure how easy it would be for me to supply the antibody (I have
the cells in the freezer, but there may be commercial complications). At least
I can give you a list of references. All of the references use the monoclonals
I described in the 1993 PNAS paper. However, the vast majority use the one used
in epitope addition experiments. The list hasn't been updated for a year, but
sure many of the references will give details for any question or problem you
have. I would be happy to update or modify the list. I you or any one else out
there has a paper not on the list, please let me know. If anyone has a paper on
the list that doesn't belong. let me know and I will remove it.
Here are the 225 references I have handy:
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