PGE assay

[Ron Walenga]

In article <Csaucier-070295010209 at 132.206.172.152> Csaucier at pharma1.pharma.mcgill.ca (John Storring) writes:

>Hi,

>I'am looking for an easy and quick protocol to measure the level of
>prostaglandin (PGE 1 or 2) released from culture cells.

>Thank you very much Caro

We have used the PGE ELISAs from Cayman Chemical Co to analyze over 30,000 
cell culture sups for PGE. THe assay is sensitive and selective enough that 
the media does NOT have to be pre-treated.  The AChE-linked technology from 
Cayman has proven superior to other labs HRP linked reagents, at least in our 
hands.