F-actin label in LIVE cells

Tobias Baskin baskin at BIOSCI.MBP.MISSOURI.EDU
Fri Feb 17 10:58:29 EST 1995


        Greetings,
                 Warren Gallin wrote:

>No-one uses phalloidin for live cells, it is essentially impermeable
>(except for hepatocytes). ...

        Indeed, Rhodamine phalloidin is impermeable. However, it is
commonly used successfuly to label actin in living cells (for example, try
Sanders and Wang 1991 J. Cell Sci. 100: 771 - 780, or Cleary et al. 1992 J.
Cell Sci. 103: 977 - 988). You do have to microinject it to get it in. Same
as for fluoresecent anti-actin. At low concentrations, it is accepted as
being a reasonable reporter of filamentous actin.
        I am sorry if the original question specified a request for a probe
that was membrane permeable.

        Hope this helps,
                        Tobias Baskin

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       ___     ____   ^         ____        _____    Tobias I. Baskin
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