Tyr Kinase Activity in PMNs

[Steven Debol MicroBio]

If anyone has used anti-phosphotyrosine antibody to measure
tyrosine kinase activity in neutrophils, and wouldn't mind
answering a few questions, I would appreciate it if you
would email me.

I am using the PY-20 antibody to immunoprecipitate phosphorylated
proteins and then using 4G10 to probe them on Western blots.  Although
my signal to noise ratio is much better than using whole cell
extracts, I seem to be missing some important proteins reported
in the literature.

Any help would be greatly appreciated.  Thanks!

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Steve Debol                       "Your picture's in my wallet and
Dept. of Microbiology              I'm sitting on it--if that isn't 
University of Minnesota            love I don't know what is."
sdebol at lenti.med.umn.edu                              --Frank Burns
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