Microinjection experts; help me out please!

Louis-Eric Trudeau letrud at iastate.edu
Tue Feb 21 11:56:40 EST 1995


umdoble at mail.cc.umanitoba.ca (Brad Doble) wrote:
>I am trying to inject cultured neonatal rat cardiac myocytes, and have a
>problem with the microinjection pipette surging forward when I press the
>inject button (often leading to ruined pipettes!).  This happens even when
>the injection pressure (as displayed on the LED readout of the injector)
>is only 1 psi.  Does anyone know what might be going wrong?   I am using a
>fluorescent dye to monitor the delivery of the injectant and thus, know
>that the 1 micrometer diameter tips of the pipettes are not clogged.
>
>Any feedback would be greatly appreciated.
>
>Thanks in advance,
>
>Brad
>
>--
>Brad  Doble                    umdoble at mail.cc.umanitoba.ca
>                                  or:  bradd at sbrc.umanitoba.ca
>Department of Physiology, University of Manitoba




One possibility is that the diameter of the opening of your capillary holder is too large for the O.D. of your pipettes.
Another possibility is the clamping of your pipette in the holder: is there a suitable O-ring there?
Good luck.

Louis-Eric
Laboratory of cellular signaling
Iowa State University



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