E.coli cell breaking

EMMERLING,MARCEL GRUEMSCHI at EZINFO.VMSMAIL.ETHZ.CH
Mon Jan 2 06:06:38 EST 1995


I want to determine intermediate products of E.coli (PEP, Pyr, G6P, etc). I
tried to break my cells with perchloric acid and KOH treatment, but i finally
found out, that some products are acid instable. Is there any method of
breaking cells without acid.


Thanx,

	Marcel
gru at ibtech.biol.ehtz.ch




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