gene targeting in cell type other than ES

[Riccardo Fodde]

Hi everybody,

I would like to ask for information on gene targeting in EBV-transformed 
celllines.  Has anybody tried this and how to get single colonies?
Also I would like to know if it is possible to target primary fibroblasts 
without having to immortalize them first and still have enough passages left 
to do something with correctly targeted cells.

Thanks,                               Joris Heus 

E-mail:       JHEUS at RULY46.LEIDENUNIV.NL