FITC conjugation of purified proteins
kruskal at a1.tch.harvard.edu
Fri Jan 6 12:16:51 EST 1995
FITC conjugation is generally fairly straightforward and should not
require a kit. The most important points are:
1. Use the best quality FITC you can get (I recommend either
4353 E. 49th St.
Cleveland, OH 44125 or
4849 Pitchford Av.
Eugene, OR 97402).
2. The unreacted FITC tends to be hard to separate, and for most
purposes it's important to remove it. I've usually used extensive
dialysis, although many protocols recommend gel filtration over
Reasonable protocols include:
Harlow & Lane, Antibodies: A Laboratory Manual, 1988, Cold Spring
Hope this is helpful.
More information about the Cellbiol