HeLa cell extracts for SV40 DNA replication

wick at cris.com wick at cris.com
Tue Jan 10 17:50:07 EST 1995

Does anyone have a good procedure for preparation and storage of HeLa cell extracts for use in SV40 DNA replication. We have 
tried the method of Li and Kelly and that of Wobbe and Hurwitz. The initial activities are sometimes OK but are rapidly lost when 
stored at -70C.

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